SEA chain exchange amplification technology to detect the performance index of Listeria monocytogenes

Strand Exchange Amplification (SEA) is a new generation of isothermal nucleic acid detection technology with independent intellectual property rights. It is simple, fast, economical, highly specific, sensitive, and highly expandable. The characteristics have broad application prospects in the fields of medical health, food safety, inspection and quarantine, environmental monitoring, animal diseases and the like.

Listeria monocytogenes is a common soil bacterium in which it is a saprophytic bacterium that feeds on dead and decaying organisms. It is also a contaminant in certain foods (mainly fresh milk products) that can cause severe food poisoning. Listeria monocytogenes is a pathogen of zoonotic diseases. It can cause Lie's disease in humans and animals. After infection, it mainly manifests as sepsis, meningitis and mononuclear cells, which is a serious threat to human health. Therefore, the detection of Listeria monocytogenes in food is particularly important.

Using SEA technology to study Listeria monocytogenes, the team developed a rapid detection kit for the detection of single increase , and published the results in Anal, Biochem. 2018, 545: 38-42 . High specificity, detection limit of 1.0 × 10 -14 M, good repeatability, and can be used commercially.

Specificity and anti-interference ability

A: The specificity of the SEA method. 1-6 are respectively expressed as: based on the enrichment liquid of Listeria monocytogenes, Staphylococcus aureus, Salmonella typhimurium, Vibrio parahaemolyticus, Bacillus cereus and Escherichia coli O157:H7 1000 times later is the amplification target; 7 represents NTC.

B: Anti-interference ability of the SEA method. 1ËŠ indicates that the target is a 1000-fold dilution of Listeria monocytogenes; 2ËŠ indicates a mixture of targets, ie, Listeria monocytogenes, Staphylococcus aureus, Salmonella typhimurium, parahemolytic Vibrio vibrio, Bacillus cereus and E. coli O157:H7 were diluted 1000 times and mixed in a ratio of 1:1:1:1:1:1. 3ËŠ indicates NTC.

Detection limit and repeatability

A: Real-time fluorescence curves of different concentrations of Listeria monocytogenes genomic DNA. 1-5 is expressed as 1.0 × 10 -10 to 1.0 × 10 -14 M, diluted 10 times, and 6 represents NTC.

B: Relationship between the T t value and the negative logarithmic value of the genomic DNA target amount (C LM ). The error bars show the mean standard deviation of the three measurements.

C: The corresponding SEA reaction product was observed using PAGE electrophoresis.

In addition, our company has a series of nucleic acid detection kits in the food safety field, such as Staphylococcus aureus, Escherichia coli O157:H7, Vibrio parahaemolyticus, Bacillus cereus, etc. The provincial and municipal food and drug supervision bureaus have performed well in blind sampling tests. They have signed strategic cooperation agreements with well-known domestic food companies and obtained good customer response. Customers and distributors are welcome to purchase.

Smart Doorphone With Tuya

We have a variety of designs for Intercom System With Tuya, different sizes, different keys, etc., you can refer to our pictures,

We have been focusing on independent research and development of intercom system with tuya

since 2011. We have professional reach and devolve team to serve you, and we can provide you with professional products and services, If you need more information about our factory or intercom system, please do not hesitate to contact us, we are very honored to share catalog with you.

Smart doorphone with tuya is an intercom system that can be connected to a mobile phone. The system adopts a unified TCP/IP networking architecture to connect all-digital video intercom, security equipment and elevator linkage system. At the same time, it can be connected to the central management machine. The property community can better manage tenants, improve user experience and property services, etc. They use POE switches to connect multiple indoor units and video door phones, and POE switches supply power to the indoor units. The wiring is very simple and convenient, and the overall cost is low.

Smart doorphone with tuya improves user experience, because users can receive call information, and can monitor Video Door Phone at any time through tuya application, even if users are in an office, picnic or travel, they can immediately receive call information from visitors , and talk to them, you can also monitor visitors. If it is the user's friend, it can also remotely unlock the visitor on the mobile phone, which is very convenient.

The video doorphone in the picture is the most popular model from our company - MK-AZZJ4C, we have adopted the embedded design, which is very classic and beautiful. It is also an Android system, which can support simultaneous connection of multiple indoor units and mobile phones to realize remote monitoring, calling and unlocking on the mobile phone. We have also added the function of face recognition and LED fill light, which can clearly monitor and unlock using face recognition even at night. Ideal for multi-apartment, hotel and community applications.

Smart doorphone with tuya

The following are the parameters of MK-AZZJ4C for your reference:


1. 4.3 inch 720 HD display TCP/IP Intercom system

2. Calling, intercom, unlocking, monitoring

3. High quality aluminium alloy frosted panel + high-end glass lens + sensor buttons

4. Digital camera, stable and clear image shooting

5. Night vision and bright light compensation function

6. Full duplex voice intercom, comparable to ordinary telephone intercom

7. Android system ,ARM CPU, TUYA App (support Android and IOS system)

8. LPDDR3 1 GB memory, standard EMMC 8 GB storage expandable

9. RJ45 standard ethernet interface all-digital design

10. Low power consumption technology solution, saving energy and long life span

11. Dimension:400*140*51(mm)

12. Install method : Wall/flush install (390*130*46 mm)

13. Image/video advertising playback (advertising can be customized)

14. Door opening:IC/ID card/ password/ face recognition/ mobile phone

Our company has been focusing on intercom system for 11 years, we can provide you with different systems, like IP, 2 wire and 4 wire system, and we are also OEM & ODM factory, support to customize your brand and function, Thank you very much for your interest in our products, if you need more detailed information, please feel free to contact me.

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Zhuhai Mingke Electronics Technology Co., Ltd , https://www.zhmkdz-electronics.com