1, the choice of varieties of thin-skinned melon varieties are much easier to choose, such as thin-skinned melon; very sweet melon, Xinfu jade, Wulong sugar king, etc. are deeply loved by melon farmers. 2, cultivation system Melon is a warm and heat-resistant crop, melon avoids continuous cropping, continuous cropping is easy to cause blight and other soil-borne diseases. Melon should not be used in conjunction with other melon crops to avoid getting sick. The melons are mature early, the growth period is short, the plant spacing is large, and the economic benefits are relatively good. It is a rational crop for the Datian agricultural area and the grain, cotton and oil crops. 3. It is very important to choose the land for land preparation. The roots of melon are more developed, drought-tolerant and not wet. The melons should be well-drained, and the sandy loam with deep soil layer should be suitable. The choice of plots should be based on leeward sunny, high-lying terrain, no drought, and landfills that can be replenished. Land preparation, tillage is best done once in winter and spring. After the spring soil is frozen, the spring cultivation is carried out. Most of the melon fertilization is applied to the base fertilizer. The base fertilizer can be applied in two times; the first time is the surface application, that is, the composting, grass manure and other coarse fertilizers are fully applied before the spring ploughing, and the fertilizer is turned over into the layer; the other time is the hole application, before sowing. Dig a well under the seeding hole to apply fully fermented manure, cake fertilizer, manure or compound fertilizer. After applying the base fertilizer, you can make a sputum and mulch. Melon has a long drought and a lot of rain in the late stage. In order to balance the need of drought prevention and irrigation, it is often made into a low ridge. The effect of early maturity, yield increase and income increase of melon mulching film cultivation is very significant. The promotion of this technology has developed rapidly and has become a routine measure for the high-quality and high-yield cultivation of melon. There are two ways to cover the mulch: one is to sown after filming. The other is to lay the film after sowing. 4, seeding seedlings seedlings must be arranged in the local temperature began to warm up, and has been stable through the night frost period, sowing seedlings quickly unearthed, not long-term principle. Since the surface of the kneading surface under the ground has always maintained a certain temperature and humidity, it is only necessary to broadcast it. It is advisable to cover the soil thickness without seeing the seeds. After the young shoots are unearthed, the membrane should be ruptured early, and the seedlings should be placed to prevent the growth. 5, seedling transplanting northern melon open field cultivation, usually using live broadcast: in order to premature morning market, supply the market in advance, the city suburbs also use seedling transplanting. The seedbed should be selected in the place where the leeward is sunny, the terrain is high and dry, and it is close to the water source and the planting land. The regenerative ability of melon roots is very weak. When transplanting, the roots are longer and the seedlings are longer, which affects early maturity. The nutrient soil used for seedling cultivation is prepared from 6-7 parts of the field topsoil and the hit-selling parts of the melons that have been fermented and fully fermented. The nutrient soil of each cubic meter is also mixed with calcium superphosphate. . 5 kg of grass ash, after sieving, spray 800 times of thiophanate and trichlorfon. Before sowing, the seeds are subjected to seed selection, seeding and soaking and germination. Pour the seedbed before planting. For sowing, the seedbed should be punctured first, then one germinated seed should be sown one by one, and the bud tip is placed down into the hole. Cover the soil immediately after the broadcast. Covering soil is a field topsoil that has been screened. The thickness of the covering soil is about 1 cm. Pay attention to the adjustment of temperature and humidity in the management of seedbed. Before the seedlings are unearthed, the temperature of the shed should be kept between 25 and 35 degrees. After the seeds are germinated, they should be ventilated and cooled to prevent the seedlings from promoting growth. The shed temperature is maintained at 25-28 degrees during the daytime, not less than 15 degrees at night, and 5-7 days before the seedlings are planted. The ventilation should be gradually increased to exercise the seedlings. In the last 1-2 days, the shed film can be used. All were removed to adapt the seedlings to the open environment after planting. When the night frost has passed and the ground temperature has stabilized above 15 degrees, the seedlings can be transplanted and planted. One afternoon before planting, the seedbed should be poured once. Before planting the seedlings, the holes are drilled on the planting ground according to the plant spacing. The planting holes are slightly larger than the nutrient blocks of the nursery, and the hole depth is the same as the height of the soil block. After the planting hole is laid, the seedlings with plastic enamel or paper bag can be gently put into the hole, and the soil around the nutrient block is filled with fine soil, compacted by hand to make it close to the fine soil to prevent empty seedlings. . 6. Pruning and picking is the most important and technically strong management measure for melon production. The result of melon is mainly sun vine, and there are also some vines. The direct result of the main vine is very small. Therefore, most of the varieties are top and side vines, with double or multiple vines. Double vine pruning is a widely used pruning method in the production of thin melons. Pruning method. Generally, when the seedlings of the thin melon varieties are 3 to 5 true leaves, the main vines are topped and the tops are selected. After the vines are extended, the 2 vines are selected, and the remaining vines are all removed, and the selected 2 vines are led to the glutinous rice. Spread evenly. In some places, multiple vines are used for making varieties. The mid-late ripe varieties of melons often use traditional three-vine pruning. When the seedlings are 4-8 pieces of true leaves, the main vines are topped, and then the vines protruding from the base are selected to retain 3-4 pieces of healthy vines. After the noodles, the remaining vines were removed early, and the results of the sun vines with female flowers were retained, and the vines without female flowers were removed. Top picking is also called topping, playing power, and the main point of picking the heart is: the early picking should be timely, accurate, and thorough, that is, it should be early in time and timely. The stems of melon grow very fast, and after picking up the heart, it will not adjust the balance. Experienced melon farmers often hold thin bamboo rafts and go back and forth in the melon garden. They will hit the head and hit the top. For a few days, you can get the same effect as every vine. 7, topdressing watering melons The growth period of melons is relatively short, only need to apply enough base fertilizer, generally less topdressing. Melon topdressing, watering, should master the seedling stage less top dressing, less watering, or no top dressing, no watering. In the flowering and fruiting period, in order to prevent excessive growth of nutrients and promote fruit setting, water and fertilizer should be controlled. Generally, topdressing and no watering should be used, and nitrogen fertilizer should not be used in flowering period. During the results period, multiple top dressings should be carried out, and water should be watered as appropriate to ensure that the results are fully developed and expanded. Therefore, the melon should be strictly controlled before watering, and the phosphorus and potassium fertilizer should be added. 0.1%-0.5% potassium dihydrogen phosphate solution can be used as the root dressing. 8. Mature harvesting only harvests fully ripe fruits at the right time to ensure the sweetness inherent in melon. Disclaimer: Some articles on this website are transferred from the Internet. If legal rights of third parties are involved, please inform this website. phone
PCR ( Polymerase Chain Reaction)
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It provides a way to make more copies of a portion of DNA.
We need to have PCR ingredients that include DNA portion, Buffer solution, Primer+DNA (tap polymerase), and Nucleotides during the preparation step.
In the step of PCR sequence, there are three steps in this section.
Step 1 Denaturation
The addition of heat is needed to separate the two strands of the DNA molecule.
Step 2 Annealing
Temperature, is going to be cool in this step, should allow the primers to bind to the specific segments.
Step 3 DNA Synthesis
To make more copies of the DNA.
Those three steps above keep repeating to make enough copies of the DNA fragments. It is the principle of how PCR machine works.