CO 2 incubators are widely used in medical, immunology, genetics, microbiology, agricultural science, and pharmaceutical research and production, and have become one of the most commonly used routine instruments in laboratories in these fields. The carbon dioxide incubator simulates the formation of a cell/tissue-like growth environment in the incubator. The incubator requires a stable temperature (37 ° C), a stable CO 2 level (5%), and a constant pH ( pH: 7.2-7.4), higher relative saturation humidity (95%), a device for in vitro culture of cells/tissues. In general, users have two basic requirements for a carbon dioxide incubator: First, the carbon dioxide incubator is required to provide the most accurate and stable control of temperature, carbon dioxide concentration and humidity, so as to facilitate the progress of its research work. Second, the carbon dioxide incubator is required to effectively prevent microbial contamination in the incubator and Eliminate pollution regularly to protect research results and prevent sample loss. CO 2 incubator purchase guide The CO 2 incubator is widely used, and there are many types and models of CO 2 incubators on the market. How to choose a carbon dioxide incubator suitable for cell culture? Before choosing a CO 2 incubator, you need to consider the following factors: Temperature control: air jacket heating VS water jacket heating Maintaining a constant temperature in the incubator is an important factor in maintaining healthy cell growth. When purchasing a CO2 incubator, there are two types of heating structures to choose from: air jacketed heating and water jacketed heating. The gas-sleeve heating system directly heats the gas in the tank through a heater in the tank. The air-sleeve design can quickly restore the temperature stability inside the box under the condition that the temperature caused by frequent switching of the door is frequently changed. The water jacketed incubator maintains a constant temperature by surrounding the internal tank through a separate hot water compartment. The hot water circulates through the natural convection in the tank, and the heat is transferred to the inside of the tank through the radiation to maintain a constant temperature. Although both heating systems are accurate and reliable, they all have their own advantages and disadvantages. The unique water jacket design has its advantages: water is a good thermal insulation material, and when it is powered off, the water jacket system can more reliably maintain the temperature accuracy and stability in the incubator for a long time ( The time to maintain a constant temperature is 4-5 times that of a gas-tight system). If your experimental environment is not stable (such as useful electrical limits, or frequent power outages) and you need to maintain stable culture conditions for a long time, then the water jacketed carbon dioxide incubator is your best choice. Compared with the water jacket type, the gas-sleeve type has the characteristics of quick heating and rapid recovery of the temperature than the water-type incubator, which is particularly advantageous for short-term cultivation and the need for frequent switching of the door. In addition, the air-sleeve design is simpler for the user than the water jacket type (water jacket type requires watering, emptying and cleaning of the water tank, and often monitoring the operation of the water tank). Carbon dioxide sensor: infrared sensor (IR) VS heat conduction sensor (TC) CO 2 concentration detection can be measured by two control systems, an infrared sensor (IR) or a thermal conduction sensor (TC). When the door of the carbon dioxide incubator is opened, CO 2 leaks out of the tank, and the sensor detects a decrease in the concentration of CO 2 and reacts in time to re-inject CO 2 to the original preset level. . The principle of thermal conductivity sensor (TC) monitoring CO 2 concentration is achieved by measuring the change in resistance between two thermistors (one regulator exposed to the cabinet environment and the other enclosed). Infrared sensor (IR) is another optional control system with more accurate CO 2 control than the TC system. It uses an optical sensor to detect CO 2 levels. The IR system includes an infrared emitter and a sensor. When the CO 2 in the tank absorbs part of the infrared light emitted by the emitter, the sensor can detect the amount of infrared radiation, and the amount of absorbed infrared light corresponds to the CO 2 in the tank. The level so that the concentration of CO 2 in the tank can be derived. One disadvantage of the TC control system is that changes in temperature and relative humidity within the chamber can affect the accuracy of the sensor. When the door is frequently opened, not only the CO 2 concentration, but also the temperature and relative humidity fluctuate greatly, thus affecting the accuracy of the TC sensor. This control system is less suitable when precise culture conditions are required and the incubator door is frequently opened. Because the IR system is not affected by changes in temperature and relative humidity, it is more accurate than the TC system and is especially suitable for cell cultures that require frequent opening of the incubator door. However, this system is more expensive than the TC system, and it is necessary to consider the budget. Convection system: forced convection vs natural convection The so-called natural convection is the natural flow of air by the natural flow of airflow (hot air rise, cold air drop, natural wind, etc.). The so-called forced convection is the use of some fans, heating (or cooling) equipment, specially designed air ducts (or pipes) to make the air flow in a specific environment. There are generally components of manual intervention in forced convection. In general, natural convection requires very low equipment and is the most basic method of convection. However, in order to enhance the air flow effect, the equipment is usually made of high-quality stainless steel plate and has a duct, and a fan is used to form forced convection. Improve the uniformity of temperature in the incubator and the uniformity of CO 2 concentration. Recovery rate and accuracy of parameters after opening the door Easy-to-use and user-friendly control system Internal cavity environmental quality and cleanliness Automatic circulation sterilization system Excellent performance and quality The cabinet can be stacked to save space Optional spare parts The product passed the factory performance test and international standard certification. The above are some factors to consider when purchasing an ideal CO 2 incubator. Please be sure to consider the full range. I hope you can choose a suitable CO 2 incubator! Esco's full range of carbon dioxide incubators are the perfect cradle for your cell culture, providing optimal culture conditions for all types of cells, and providing comprehensive performance for your cells with their reliable performance, simple operation and perfect design. Protection to help you achieve your scientific dreams! Natural sweeteners refer to food additives that can sweeten soft drinks. 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