YIJI protein sample resin detoxification kit The main purpose YIJI Protein Sample Resin De-endotoxin reagent is an authoritative and classical technical method aimed at removing bacterial endotoxin from samples by filtration and adsorption by lipopolysaccharide affinity resin. The technology has been carefully developed and successfully tested. It is suitable for the preparation of various bacterial protein purified samples. The product can be used in a variety of subsequent animal and cell biology operations. The product is ready to use, stable performance, convenient operation and satisfactory cleaning efficiency. technical background Endotoxin is a component of Lipopolysaccharides (LPS), a cell membrane of Gram-negative bacteria. During the purification of bacterial proteins, including fusion proteins, bacterial lysis releases endotoxin, accompanied by proteins that severely interfere with subsequent sensitive animal and eukaryotic cell experiments. YIJI protein sample resin detoxification technology incorporates lipopolysaccharide-specific affinity resin, namely polylysine coated and functionalized cation exchange groups, ultra-highly crosslinked polystyrene polymerization. Hyper-crosslinked polystyrene polymers are sufficiently effective to filter and remove endotoxin in the sample by 10 μg/ml, ie 2500 to 5000 EU/ml. Non-specific protein binding by chromatography, loss of immunological activity, and partial endotoxin failure and expensive disadvantages are avoided. product content YIJI Affinity Liquid (Reagent A) 3 ml Product manual 1 copy storage method Stored in a 4 ° C refrigerator, effective for June User-supplied 1.5 ml centrifuge tube: container for sample handling Mini Tabletop Centrifuge: for Precipitating Affinity Resins Vortex oscillator: used to mix reactants Flat shaker: used to mix reactants Experimental procedure Before the start of the experiment, the sample of the protein to be tested was taken out from the -70 ° C refrigerator and thawed in an ice bath. Then do the following. Precautions Quality Standard Food Acidulants,Free Xanthan Gum,Guar Gum,Citric Acid Monohydrate Shanghai J.Shine Co.,Ltd , https://www.jshinechem.com